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IBI GEL/PCR DNA Fragment Extraction / Clean Up Sample Kit - 4 PREPS
The Gel/PCR DNA Fragment Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. The contaminants are washed with a wash buffer (containing Ethanol) and the purified DNA fragments are eluted by a low salt based elution buffer or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are 80-90% for Gel Extraction and 90-95% for PCR Clean-Up. The entire procedure can be completed in 20 minutes, and the eluted DNA is ready to use in restriction digestion, ligation, PCR, and sequencing reactions. KEY FEATURES SAMPLE SIZE: up to 300mg of agarose gel / up to 100μl of PCR products FORMAT: Spin Column OPERATION: Centrifuge / Vacuum Manifold BINDING CAPACITY: 10μg DNA EXPECTANT YIELD: 80-90% for gel extraction / 90-95% for PCR Clean-Up OPERATION TIME: 20 minutes or less APPLICATIONS: PCR, Fluorescent or Radioactive Sequencing, Restrictive Digestion, DNA Labeling, Ligation KIT CONTENTS Component IB47010 IB47020 IB47030 DF COLUMNS 4 100 300 2ML COLLECTION TUBES 4 100 300 DF BUFFER 3ml 80ml 240ml WASH BUFFER 1ml 25ml 50ml (add Ethanol) (4ml) (100ml) (200ml) ELUTION BUFFER 1ml 6ml 30ml ORDERING INFORMATION CATALOG# DESCRIPTION IB47010 GEL/PCR FRAGMENT EXTRACTION SAMPLE KIT-4 PREPS
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