Recenttec IEF Do-It-Right is a new and convenient way to measure salt content in 2-DE samples, and then suggest a total voltage hour for your IEF experiment.
Two-dimensional electrophoresis (2-DE) is widely used for proteomic researches. In the first dimensional IEF, salt interference is thought to be a major factor which deteriorates 2-DE results. Salt contents in 2-DE samples may result in high heat and high conductivity, this may burn the immobilized pH gradient (IPG) gel strips. In IEF, the supplied electric energy, in terms of volt-hours (Vh), should be optimized to avoid underfocusing or overfocusing. Now, IEF Do-It-Right combines a IEF Salt Meter with an on-line software (Voltage-Hour Calculator), this product can predict an optimal IEF total voltage hour for your IEF experiments. The reproducibility and the success rate of 2-DE experiments can be greatly increased.
• IEF Salt Meter provides an accurate salt content surement of 2-DE samples • Voltage-Hour Calculator provides optimal IEF total voltage hour for 2-DE samples • Increases the reproducibility and successful rate of 2-DE experiments