The transfer unit stack is formed by placing the gel and membrane in contact, sandwiched between filter papers soaked in transfer buffer. An electric current is applied perpendicular to the gel and buffer-saturated filter papers, allowing the bands from the charged molecules of the gels to migrate and bind to the membrane, completing the transfer. The transfer time depends on molecule size and the number of transfer units. Multiple stacks can be run simultaneously for efficient Western Blot processing.
Additional Details:
Ideal for efficient and economical transfer of protein, RNA, or DNA from polyacrylamide or agarose gels to a solid membrane support, which is subsequently visualized by staining methods.
Working Surface Dimensions:
– Semi-Dry Blotter: 16cm x 16cm
Recommended Maximum Capacity:
– Semi-Dry Blotter: 1 – 6 trans-units
Both the Semi-Dry Blotter and Semi-Dry Blotter Large have graphite-coated plates to reduce heat, minimize buffer usage, and produce clear, undistorted transfer bands.
A transfer unit stack is formed by placing the gel and membrane in contact, sandwiched between filter papers soaked in transfer buffer. An electric current is applied perpendicular to the gel and buffer-saturated filter papers, allowing the bands from the charged molecules of the gels to migrate and bind to the membrane, completing the transfer. The transfer time depends on molecule size and the number of transfer units.
Several stacks of transfer units can be run simultaneously:
– The Semi-Dry Blotter can accommodate six trans-units at the same time.
Specifications:
Semi-Dry Blotter
Working surface dimensions: 16.0cm(W) x 16cm(L)
Weight: 3.75 lbs.
Cathode electrode wire: 36 inches
Anode electrode wire: 36 inches
Recommended capacity:Â 1 – 6 trans-units.
Electrode plate material: graphite
Operations Manual Link:
IB44000/IB45000 – Semi-Dry Blotter Operation Manual
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